Start-up is a very small, beginning enterprise that has a business idea, but it is almost lacking all the resources needed to develop a business, including experiences. Extremely fast growth and scalability cannot be ensured by internal resources from organic growth, so start-up will be dependent on an external support. The aim of the research was to find out how start-ups perceive the form and degree of external support from larger and established companies, government/state administration and universities, start-up scene and investors. Research has shown that the most effective and most accepted support is provided by the start-up scene and investors, apparently less support comes from larger established enterprises, support from the state/government and universities is little expected and accepted. The key to suitable support is to investigate the motivation of providers and recipients of support and to understand the asymmetry of their relationships. The extent and quality of start-up support from larger and established companies depends on mutual expectations. Support of start-ups from government and higher education institutions has the lowest effect among all external actors. Support from investors is accepted most among the evaluated impacts. Investors with their resources are a vital condition for running the start-up.
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